Standards for rapid testing of Pseudomonas aeruginosa in packaged drinking water officially implemented

Generally speaking, the testing of packaged drinking water mainly involves microorganisms, quality indicators, contaminant limits, labeling, etc. Among them, a statistical analysis of microbial unqualified items showed that Pseudomonas aeruginosa has a relatively high unqualified rate, making it a very prominent quality and safety issue for packaged drinking water.

In order to ensure the quality and safety of packaged drinking water, on the one hand, packaged drinking water companies must ensure the quality and safety of production, on the other hand, they must strengthen sampling inspections of packaged drinking water to prevent problematic products from flowing into the market.

It is understood that "DB64/T1682-2019 Rapid Detection Method for Pseudomonas aeruginosa in Packaged Drinking Water" was released on December 18, 2019, and was released on March 17, 2020. officially implemented on the day. This standard was proposed by the Ningxia Hui Autonomous Region Food Inspection Research Institute, and is organized and implemented by the Ningxia Hui Autonomous Region Market Supervision and Administration Department. There are two main drafting units, including the Ningxia Hui Autonomous Region Food Testing Research Institute and the Ningxia Hui Autonomous Region Food and Drug Evaluation and Inspection Center.

In addition, "DB64/T1682-2019 Rapid Detection Method of Pseudomonas aeruginosa in Packaged Drinking Water" mainly stipulates the detection of Pseudomonas aeruginosa in packaged drinking water and drinking natural mineral water. The loop-mediated isothermal nucleic acid amplification method is suitable for the qualitative detection of Pseudomonas aeruginosa in packaged drinking water and drinking natural mineral water.

In addition, the standard also provides testing specifications in terms of reagent materials, instruments and equipment, testing procedures, operating steps, waste disposal, etc.

As far as instruments and equipment are concerned, the standard stipulates that instruments and equipment include constant temperature incubators, high-speed desktop centrifuges, fluorescence quantitative PCR instruments, etc. In the operating steps, the standards set out standardized requirements in terms of sample preparation and enrichment culture, preparation of template DNA, loop-mediated isothermal nucleic acid amplification, and result judgment.

Among them, the preparation of template DNA includes two aspects, and the loop-mediated isothermal nucleic acid amplification includes five aspects, namely amplification reagent preparation, sample addition, reaction process, quality control, etc. wait.

It is worth mentioning that in the reactionProcess requirements, "DB64/T1682-2019 Rapid Detection Method of Pseudomonas aeruginosa in Packaged Drinking Water" stipulates that the process should be carried out in the amplification area, and the centrifuged PCR reaction tube should be placed into the fluorescence quantitative PCR detection system at a constant temperature of 63°C. Amplify for 45 minutes, and the reaction program is 63°C for 15s and 63°C for 45s as one cycle, a total of 45 cycles. After the detection is completed, the results are determined based on the amplification curve and peak time.

Afterwards, the test results of the sample are mainly judged based on the amplification curve and peak time. According to the new standard, if the sample to be tested has an "S"-shaped amplification curve, the result will be positive. On the contrary, if there is no "S"-shaped amplification curve, or a straight line or an inclined straight line, and the detected peak time is 0, the test result of the sample is judged to be negative.

Of course, the implementation of "DB64/T1682-2019 Rapid Detection Method of Pseudomonas aeruginosa in Packaged Drinking Water" is undoubtedly the rapid detection of Pseudomonas aeruginosa in packaged drinking water. It provides a standard basis, improves detection efficiency and accuracy, and prevents problematic products from flowing to the market in a timely manner. In addition, it is necessary to strictly control every key link in the production of packaged drinking water, from in-factory production and processing to outside the factory, and strengthen control in circulation and sales. A multi-pronged approach is needed to avoid the contamination of packaged drinking water by microorganisms in the environment. , to ensure the safety of drinking water for consumers.

　(Original title: Relevant standards for rapid testing of Pseudomonas aeruginosa in packaged drinking water are officially implemented today)